|Application ||WB, IHC, E|
|Preparation||Produced from sera of rabbits pre-immunized with highly pure recombinant Murine RELMα. Anti-Murine RELMα specific antibody was purified by affinity chromatography employing immobilized Murine RELMα matrix.|
|WesternBlot||To detect Murine RELMα by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMα is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich||To detect Murine RELMα by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine RELMα (61-115BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELMα.|
|Immunohistochemistry||This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse). The primary antibody was incubated at 4 ng/mL overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.|
*Additional Immunostaining data available. Please contact Tech Support for information.
|Formulation||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Murine RELMα Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.