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Anti-Murine RELMβ Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Additional Information
Preparation Produced from sera of rabbits pre-immunized with highly pure recombinant Murine RELMβ. Anti-Murine RELMβ specific antibody was purified by affinity chromatography employing immobilized Murine RELMβ matrix.
WesternBlot To detect Murine RELMβ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMβ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich To detect Murine RELMβ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine RELMβ (61-116BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELMβ.
Immunohistochemistry This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4°C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was used. Information and photos are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.
Formulation A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage-20°C
PrecautionsAnti-Murine RELMβ Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 275.00
$ 216.00
Cat# ABG10477
Size:
Quantity:
(40 western blots)
Availability: 5-7days
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