Anti-Human sCD40 Ligand Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
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Reactivity | Human |
Host | Goat |
Clonality | Polyclonal |
Preparation | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L. Anti-Human sCD40L specific antibody was purified by affinity chromatography employing immobilized hsCD40L matrix. |
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WesternBlot | To detect hsCD40-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40-Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich | To detect hsCD40-Ligand by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human sCD40-Ligand (60-101) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40-Ligand. |
Immunohistochemistry | This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentrations are 2.5 μg/ml - 5.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. The recommended antigen retrieval is heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Anti-Human sCD40 Ligand Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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