Biotinylated Anti-Human sRANK Ligand Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsRANKL. Anti-Human sRANKL specific antibody was purified by affinity chromatography and then biotinylated. |
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WesternBlot | To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich | To detect hsRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human sRANKL (60-283P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsRANKL. |
Direct | To detect hsRANKL by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsRANKL. |
Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Storage | -20°C |
Precautions | Biotinylated Anti-Human sRANK Ligand Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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