|Application ||WB, IHC, E|
|Preparation||Produced in BALB/c mice using highly pure recombinant human TNF-α as the immunizing antigen. This antibody was purified from cell culture by Protein G affinity chromatography.|
|WesternBlot||To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.|
|Sandwich||In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human TNF-α when used with BioGems' biotinylated antigen affinity purified anti-Human TNF-α (60-325BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.|
|Immunohistochemistry||This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 15.0-20.0 μg/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. |
*Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
|Neutralization||To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-α (0.5 ng/ml), a concentration of < 0.1 μg/ml of this antibody is required.|
|Formulation||A sterile filtered antibody solution was lyophilized from PBS.|
|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
|Precautions||Anti-Human TNF-α Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.