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Anti-Caldesmon (Smooth) Antibody (Monoclonal, hHCD)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | Q62736 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human, Mouse |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Mouse IgG monoclonal antibody for Caldesmon (smooth), caldesmon 1 (CALD1) detection. Tested with WB, IHC-P in Human;mouse;rabbit. No cross reactivity with other proteins. |
| Gene ID | 25687 |
|---|---|
| Other Names | Non-muscle caldesmon, CDM, L-caldesmon, Cald1 |
| Calculated MW | 60584 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, mouse, rabbit, By Heat Western blot, 0.25-0.5 µg/ml, Human, mouse, rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). . |
| Tissue Specificity | High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. |
| Protein Name | Caldesmon |
| Contents | Each vial contains 50% glycerol, 1.2% Sodium acetate, 1% BSA, 0.02% NaN3. |
| Clone Names | hHCD |
| Immunogen | Human uterus smooth muscle extract. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing. |
| Name | Cald1 |
|---|---|
| Function | Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration. |
| Cellular Location | Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P13505}. Cytoplasm, myofibril {ECO:0000250|UniProtKB:P13505}. Cytoplasm, cytoskeleton, stress fiber {ECO:0000250|UniProtKB:P13505}. Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) {ECO:0000250|UniProtKB:P13505} |
| Tissue Location | High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon(h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon(l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al.(1992) demonstrated that the human CDM gene is composed of 14 exons.
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