Anti-CD4 Antibody (Monoclonal, CA-4)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-F |
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Primary Accession | P05540 |
Host | Mouse |
Isotype | Mouse IgG1 |
Reactivity | Human |
Clonality | Monoclonal |
Format | Lyophilized |
Description | Mouse IgG monoclonal antibody for CD4, CD4 molecule (CD4) detection. Tested with IHC-F in Human. No cross reactivity with other proteins. |
Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
Gene ID | 24932 |
---|---|
Other Names | T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen, CD4, Cd4 |
Calculated MW | |immunology|adaptive immunity|t cells| stem cells|hematopoietic progenitors|lymphoid|t lymphocytic lineage|myeloid|dendritic cell lineage|monocytic lineage| immunology|regulatory t cells|immune system diseases|antiviral signaling|hiv-related |
Application Details | Immunohistochemistry(Frozen Section), 1 µg/ml, Human |
Subcellular Localization | T-cell surface glycoprotein CD4 |
Tissue Specificity | 51438 MW |
Source | Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. |
Protein Name | Membrane; Single-pass type I membrane protein. |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
Clone Names | CA-4 |
Immunogen | CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. |
Purification | Ascites |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | Cd4 |
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Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T- helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. |
Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P01730}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P01730} Note=Localizes to lipid rafts. {ECO:0000250|UniProtKB:P01730} |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD stands for cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4
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