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Anti-Laminin Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P15800
Host Mouse
Isotype Mouse IgG1
Reactivity Human, Pig
Clonality Monoclonal
Format Lyophilized
Description Mouse IgG monoclonal antibody for Laminin, laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2 (laminin S) (LAMA1; LAMA2; LAMB1; LAMB2) detection. Tested with WB, IHC-P in Human;feline;pig. No cross reactivity with other proteins.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Additional Information
Gene ID 25473
Other Names Laminin subunit beta-2, Laminin chain B3, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta, Lamb2
Calculated MW 196474 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, feline, pig, By Heat

Western blot, 0.25-0.5 µg/ml, Human, feline, pig
Subcellular Localization Secreted, extracellular space, extracellular matrix, basement membrane.
Tissue Specificity Found in the basement membranes (major component). S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.
Protein Name Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clone Names LAM-89
Immunogen Human laminin.
Purification Ascites
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Contains 17 laminin EGF-like domains.
Protein Information
Name Lamb2
Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Cellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Location Found in the basement membranes (major component). S-laminin is concentrated in the synaptic cleft of the neuromuscular junction
Citations (0)

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Background

Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2(LAMA2). This gene is over 260,000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.

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$ 280.00
Cat# ABO10443
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