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Anti-Cadherin-2/N-Cadherin Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-F |
|---|---|
| Primary Accession | Q9Z1Y3 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for N-Cadherin, cadherin 2, type 1, N-cadherin (neuronal) (CDH2 ) detection. Tested with WB, IHC-F in Human;mouse;rat;rabbit;chicken. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 83501 |
|---|---|
| Other Names | Cadherin-2, Neural cadherin, N-cadherin, CD325, Cdh2 |
| Calculated MW | 99686 MW KDa |
| Application Details | Immunohistochemistry(Frozen Section), 4 µg/ml, Human, mouse, rat, rabbit, chicken, - Western blot, 2 µg/ml, Human, mouse, rat, rabbit, chicken |
| Subcellular Localization | Cell membrane ; Single-pass type I membrane protein . |
| Tissue Specificity | In testis, expressed in Sertoli and germ cells. . |
| Protein Name | Cadherin-2 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | GC-4 |
| Immunogen | Affinity purified chicken heart A-CAM. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Name | Cdh2 |
|---|---|
| Function | Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P15116}; Single-pass type I membrane protein. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:P15116}. Cell junction {ECO:0000250|UniProtKB:P15116}. Cell surface {ECO:0000250|UniProtKB:P15116}. Cell junction, desmosome. Cell junction, adherens junction {ECO:0000250|UniProtKB:P15116}. Note=Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes (By similarity). Colocalizes with OBSCN at the intercalated disk and sarcolemma in cardiomyocytes (By similarity). {ECO:0000250|UniProtKB:P15116} |
| Tissue Location | In testis, expressed in Sertoli and germ cells. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
N-cadherin(NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule(L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene(including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.
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