|Reactivity||Human, Mouse, Rat|
|Description||Mouse IgG monoclonal antibody for Talin, talin 1; talin 2 (TLN1; TLN2) detection. Tested with WB in Human;mouse;rat;chicken. No cross reactivity with other proteins.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Calculated MW||271684 MW KDa|
|Application Details||Western blot, 2-4 µg/ml, Human, mouse, rat, chicken|
|Subcellular Localization||TLN1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface Cell junction, focaladhesion Note: Colocalizes with LAYN at the membraneruffles. Localized preferentially in focal adhesions thanfibrillar adhesions (By similarity). |Tln1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface |TLN2: Cell junction, focal adhesion. Celljunction, synapse. Cell membrane; Peripheral membrane protein;Cytoplasmic side. Cytoplasm, cytoskeleton. Note: Focal adhesionplaques and synapses.|Tln2: Cell junction, focal adhesion Cell junction, synapse Cell membrane Cytoplasm, cytoskeleton Note: Focaladhesion plaques and synapses.|
|Protein Name||Talin-1; Talin-2|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Purified chicken gizzard talin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Contains 1 FERM domain.|
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Provided below are standard protocols that you may find useful for product applications.
Talin is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Talin serves a function of linking vinculin to the integrins, and, thus, the cytoskeleton to extracellular matrix(ECM) receptors. It has a mass of 270 kD and shares 23% N-terminal identity with ezrin, which has similar functions. TLN gene is mapped to 9p.
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