|Application ||WB, IHC-P, IHC-F, ICC|
|Description||Mouse IgG monoclonal antibody for Tenascin, tenascin N (TNN) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Calculated MW||173250 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 2-4 µg/ml, Human, By Heat|
Immunocytochemistry , 1 µg/ml, Human, -
Immunohistochemistry(Frozen Section), 2-4 µg/ml, Human, -
Western blot, 1-2 µg/ml, Human
|Subcellular Localization||TNN: Secreted, extracellular space, extracellularmatrix.|Tnn: Secreted, extracellular space, extracellularmatrix.|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the tenascin family.|
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Provided below are standard protocols that you may find useful for product applications.
The tenascins are a family of extracellular matrix proteins(ECMs). Tenascin is an extracellular matrix protein. It expressed in an unusually restricted pattern during embryogenesis and has been implicated in a variety of morphogenetic phenomena. Human hexabrachion gene(tenascin) is mapped to chromosome 9, bands q32-q34. The coding region of the hexabrachion gene spans approximately 80 kilobases of DNA and consists of 27 exons separated by 26 introns.
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