|Application ||WB, IHC-F|
|Description||Mouse IgG monoclonal antibody for Tropomyosin (36kD) detection. Tested with WB, IHC-F in Human;rabbit;chicken. No cross reactivity with other proteins.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Other Names||Tropomyosin alpha-4 chain, Tropomyosin-4, TM-4, Tpm4|
|Calculated MW||28510 MW KDa|
|Application Details||Immunohistochemistry(Frozen Section), 1-2 µg/ml, Human, chicken, rabbit, -|
Western blot, 0.5-1 µg/ml, Human, chicken, rabbit
|Subcellular Localization||Cytoplasm, cytoskeleton.|
|Tissue Specificity||TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle.|
|Protein Name||Tropomyosin alpha-1 chain|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Chicken gizzard tropomyosin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Binds to actin filaments in muscle and non-muscle cells (PubMed:7568216). Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction (By similarity). Smooth muscle contraction is regulated by interaction with caldesmon (By similarity). In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments (By similarity). Binds calcium (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton. Note=Associates with F-actin stress fibers (PubMed:7568216).|
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Provided below are standard protocols that you may find useful for product applications.
The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.
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