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>   home   >   Products   >   Primary Antibodies   >   Anti-Beta-Actin Antibody (Monoclonal, AC-15)   

Anti-Beta-Actin Antibody (Monoclonal, AC-15)

     
  • WB - Anti-Beta-Actin Antibody (Monoclonal, AC-15) ABO10494
    Anti-beta-Actin antibody (monoclonal), ABO10494, Western blottingLane 1: Rat Ovary Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: Rat Cardiac Muscle Tissue LysateLane 4: Rat Brain Tissue LysateLane 5: A453 Cell LysateLane 6: HE
  • WB - Anti-Beta-Actin Antibody (Monoclonal, AC-15) ABO10494
    Anti-beta-Actin antibody (monoclonal), ABO10494, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Brain Tissue LysateLane 4: Rat Kidney Tissue LysateLane 5: HELA Cell LysateLane 6: SMMC Cell
  • IHC - Anti-Beta-Actin Antibody (Monoclonal, AC-15) ABO10494
    Anti-beta-Actin antibody (monoclonal), ABO10494, IHC(P)IHC(P): Mouse Intestine Tissue
  • IHC - Anti-Beta-Actin Antibody (Monoclonal, AC-15) ABO10494
    Anti-beta-Actin antibody (monoclonal), ABO10494, IHC(P)IHC(P): Rat Intestine Tissue
  • IHC - Anti-Beta-Actin Antibody (Monoclonal, AC-15) ABO10494
    Anti-beta-Actin antibody (monoclonal), ABO10494, IHC(P)IHC(P): Human Intestinal Cancer Tissue
  • IHC - Anti-Beta-Actin Antibody (Monoclonal, AC-15) ABO10494
    Anti-beta-Actin antibody (monoclonal), ABO10494, IHC(P)IHC(P): Human Lung Cancer Tissue
  • IHC - Anti-Beta-Actin Antibody (Monoclonal, AC-15) ABO10494
    Anti-beta-Actin antibody (monoclonal), ABO10494, IHC(P)IHC(P): Human Mammary Cancer Tissue
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P60711
Host Mouse
Isotype Mouse IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Format Lyophilized
Description Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human;mouse;rat. No cross reactivity with other proteins.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Additional Information
Gene ID 81822
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, Actb
Calculated MW 41737 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, mouse, rat, By Heat

Western blot, 0.25-0.5 µg/ml µg/ml, Human, mouse, rat
Subcellular Localization Cytoplasm, cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
Protein Name Actin, cytoplasmic 1
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clone Names AC-15
Immunogen slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Purification Ascites
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the actin family.
Protein Information
Name Actb
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Citations (0)

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Background

The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

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$ 280.00
Cat# ABO10494
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