|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Description||Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human;mouse;rat. No cross reactivity with other proteins.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Other Names||Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, Actb|
|Calculated MW||41737 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, mouse, rat, By Heat|
Western blot, 0.25-0.5 µg/ml µg/ml, Human, mouse, rat
|Subcellular Localization||Cytoplasm, cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .|
|Protein Name||Actin, cytoplasmic 1|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the actin family.|
|Function||Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.|
|Cellular Location||Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs|
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Provided below are standard protocols that you may find useful for product applications.
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
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