|Application ||WB, IHC-P, IHC-F, ICC|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Secretogranin-3(SCG3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Secretogranin-3, Secretogranin III, SgIII, SCG3|
|Calculated MW||53005 MW KDa|
|Application Details||Immunocytochemistry , 0.5-1 µg/ml, Human, -|
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Human, Rat, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
|Subcellular Localization||Cytoplasmic vesicle, secretory vesicle lumen . Cytoplasmic vesicle, secretory vesicle membrane ; Peripheral membrane protein . Secreted. Associated with the secretory granule membrane through direct binding to cholesterol-enriched lipid rafts in intragranular conditions (By similarity). Neuroendocrine and endocrine secretory granules. .|
|Tissue Specificity||Expressed in brain, heart, kidney, liver and skeletal muscle. .|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Secretogranin 3(438-450aa DFINKQADAYVEK), different from the related mouse and rat sequences by one amino acid.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Cellular Location||Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein Secreted. Note=Associated with the secretory granule membrane through direct binding to cholesterol-enriched lipid rafts in intragranular conditions (By similarity). Neuroendocrine and endocrine secretory granules.|
|Tissue Location||Expressed in brain, heart, kidney, liver and skeletal muscle.|
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SCG3(secretogranin III) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Genetic variations in the SCG3 gene may influence the risk of obesity through possible regulation of hypothalamic neuropeptide secretion. SCG3 was the only gene within a haplotype block that contained rs3764220. SCG3 mRNA and immunoreactivity were detected in the paraventricular nucleus, lateral hypothalamic area, and arcuate nucleus, and the protein coexisted with orexin, melanin-concentrating hormone, neuropeptide Y, and proopiomelanocortin. SCG3 formed a granule-like structure together with these neuropeptides.
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