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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Calpain 1 Antibody   

Anti-Calpain 1 Antibody

     
  • WB - Anti-Calpain 1 Antibody ABO10688
    Anti-Calpain 1 antibody, ABO10688, Western blottingLane 1: HT1080 Cell LysateLane 2: COLO320 Cell Lysate
  • IHC - Anti-Calpain 1 Antibody ABO10688
    Anti-Calpain 1 antibody, ABO10688, IHC(P)IHC(P): Rat Lung Tissue
  • ICC - Anti-Calpain 1 Antibody ABO10688
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  • IHC - Anti-Calpain 1 Antibody ABO10688
    Anti-Calpain 1 antibody, ABO10688, IHC(P)IHC(P): Human Intestinal Cancer Tissue
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC
Primary Accession P07384
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 823
Other Names Calpain-1 catalytic subunit, 3.4.22.52, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, muCANP, CAPN1, CANPL1
Calculated MW 81890 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cytoplasm . Cell membrane . Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719). .
Tissue Specificity Ubiquitous.
Protein Name Calpain-1 catalytic subunit
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1(312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the peptidase C2 family.
Protein Information
Name CAPN1 (HGNC:1476)
Synonyms CANPL1
Function Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Cellular Location Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719).
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Background

Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I(mu-calpain) and calpain II(m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L(large, catalytic, 80 kD) and S(small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1(CAPN1) and L2. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy(e.g., DMD; 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS; 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CANPL1 to chromosome 11.

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$ 280.00
Cat# ABO10688
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