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Anti-Connexin 32/GJB1 Antibody
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P08034 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Gap junction beta-1 protein(GJB1) detection. Tested with WB, IHC-P in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 2705 |
|---|---|
| Other Names | Gap junction beta-1 protein, Connexin-32, Cx32, GAP junction 28 kDa liver protein, GJB1, CX32 |
| Calculated MW | 32025 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. |
| Protein Name | Gap junction beta-1 protein |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Connexin 32/GJB1(215-231aa RACARRAQRRSNPPSRK), identical to the related mouse and rat sequences. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the connexin family. Beta-type (group I) subfamily. |
| Name | GJB1 |
|---|---|
| Synonyms | CX32 |
| Function | One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Cell junction, gap junction |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells(Bergoffen et al., 1993). For a general discussion of connexin proteins, also known as Gap junction beta-1 protein. Gap junction beta-1 protein is a protein that in humans is encoded by the GJB1 gene. In melanocytic cells GJB1 gene expression may be regulated by MITF.
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