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Anti-EGF Antibody

     
  • WB - Anti-EGF Antibody ABO10721
    Anti-EGF antibody, ABO10721, Western blottingLane 1: Recombinant Mouse EGF Protein 10ngLane 2: Recombinant Mouse EGF Protein 5ngLane 3: Recombinant Mouse EGF Protein 2.5ng
  • IHC - Anti-EGF Antibody ABO10721
    Anti-EGF antibody, ABO10721, IHC(P)IHC(P): Mouse Intestine Tissue
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P01132
Host Rabbit
Reactivity Mouse
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(EGF) detection. Tested with WB, IHC-P in Mouse.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 13645
Other Names Pro-epidermal growth factor, EGF, Epidermal growth factor, Egf
Calculated MW 133072 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, By Heat
Western blot, 0.1-0.5 µg/ml, Mouse
Subcellular Localization Membrane; Single-pass type I membrane protein.
Protein Name Pro-epidermal growth factor(EGF)
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse EGF(1013-1029aa YSGDRCQTRDLRWWELR).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Contains 9 EGF-like domains.
Protein Information
Name Egf
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Background

Epidermal growth factor(EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

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$ 280.00
Cat# ABO10721
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