Anti-PPAR Alpha Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IHC-F, ICC |
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Primary Accession | P23204 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 19013 |
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Other Names | Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Ppara, Nr1c1, Ppar |
Calculated MW | 52347 MW KDa |
Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry , 0.5-1 µg/ml, Human, Mouse, Rat Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Human, Mouse, Rat Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat |
Subcellular Localization | Nucleus . |
Tissue Specificity | Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis. |
Protein Name | Peroxisome proliferator-activated receptor alpha(PPAR-alpha) |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse PPAR alpha(393-412aa NIGYIEKLQEGIVHVLKLHL), different from the human sequence by four amino acids. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR1 subfamily. |
Name | Ppara |
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Synonyms | Nr1c1, Ppar |
Function | Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn- glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2. |
Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407, ECO:0000269|PubMed:20159955} |
Tissue Location | Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis |
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Provided below are standard protocols that you may find useful for product applications.
Background
Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.
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