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Anti-PPAR Alpha Antibody

     
  • WB - Anti-PPAR Alpha Antibody ABO10734
    Anti-PPAR alpha antibody, ABO10734, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Cardiac Muscle Tissue LysateLane 4: MM231 Cell LysateLane 5: HELA Cell LysateLane 6: SMMC Cell LysateLane 7: HT1080 Cell LysateLane 8: SW620 Cell Lysate
    detail
  • IHC - Anti-PPAR Alpha Antibody ABO10734
    Anti-PPAR alpha antibody, ABO10734, IHC(P)IHC(P): Human Mammary Cancer Tissue
    detail
  • IHC - Anti-PPAR Alpha Antibody ABO10734
    Anti-PPAR alpha antibody, ABO10734, IHC(P)IHC(P): Human Mammary Cancer Tissue
    detail
  • IHC - Anti-PPAR Alpha Antibody ABO10734
    Anti-PPAR alpha antibody, ABO10734, IHC(P)IHC(P): Mouse Intestine Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC
Primary Accession P23204
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 19013
Other Names Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Ppara, Nr1c1, Ppar
Calculated MW 52347 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Immunocytochemistry , 0.5-1 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Nucleus .
Tissue Specificity Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis.
Protein Name Peroxisome proliferator-activated receptor alpha(PPAR-alpha)
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse PPAR alpha(393-412aa NIGYIEKLQEGIVHVLKLHL), different from the human sequence by four amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the nuclear hormone receptor family. NR1 subfamily.
Protein Information
Name Ppara
Synonyms Nr1c1, Ppar
Function Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn- glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407, ECO:0000269|PubMed:20159955}
Tissue Location Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis
Research Areas
Citations (0)
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Background

Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.

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$ 240.00
Cat# ABO10734
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