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Anti-Collagen IV Antibody

     
  • WB - Anti-Collagen IV Antibody ABO10840
    Anti-Collagen IV antibody, ABO10840, Western blottingLane 1: Rat Kidney Tissue LysateLane 2: Rat Lung Tissue Lysate
    detail
  • IHC - Anti-Collagen IV Antibody ABO10840
    Anti-Collagen IV antibody, ABO10840, IHC(F)IHC(F): Rat Kidney Tissue
    detail
  • IHC - Anti-Collagen IV Antibody ABO10840
    Anti-Collagen IV antibody, ABO10840, IHC(P)IHC(P): Mouse Lung Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC
Primary Accession P08572
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Collagen alpha-2(IV) chain(COL4A2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 1284
Other Names Collagen alpha-2(IV) chain, Canstatin, COL4A2
Calculated MW 167553 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Mouse, Rat, Human
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Secreted, extracellular space, extracellular matrix, basement membrane.
Protein Name Collagen alpha-2(IV) chain
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen IV(1697-1712aa LIRTHISRCQVCMKNL), identical to the related rat and mouse sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the type IV collagen family.
Protein Information
Name COL4A2 (HGNC:2203)
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Cellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Research Areas
Citations (0)
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Background

Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.

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$ 280.00
Cat# ABO10840
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