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Anti-MCM3 Antibody

     
  • WB - Anti-MCM3 Antibody ABO10966
    Anti-MCM3 antibody, ABO10966, Western blottingAll lanes: Anti MCM3 (ABO10966) at 0.5ug/mlLane 1: NIH3T3 Whole Cell Lysate at 40ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 91KDObserved bind size: 91KD
  • IHC - Anti-MCM3 Antibody ABO10966
    Anti-MCM3 antibody, ABO10966, IHC(P)IHC(P): Human Lung Cancer Tissue
  • IHC - Anti-MCM3 Antibody ABO10966
    Anti-MCM3 antibody, ABO10966, IHC(P)IHC(P): Rat Intestine Tissue
  • IHC - Anti-MCM3 Antibody ABO10966
    Anti-MCM3 antibody, ABO10966, IHC(P)IHC(P): Rat Intestine Tissue
  • IHC - Anti-MCM3 Antibody ABO10966
    Anti-MCM3 antibody, ABO10966, IHC(P)IHC(P): Mouse Intestine Tissue
  • ICC - Anti-MCM3 Antibody ABO10966
    Anti-MCM3 antibody, ABO10966, ICCICC: HELA Cell
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession P25205
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM3(MCM3) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 4172
Other Names DNA replication licensing factor MCM3, 3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3
Calculated MW 90981 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Immunocytochemistry , 0.5-1 µg/ml, Human, -
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Nucleus.
Protein Name DNA replication licensing factor MCM3
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3(13-27aa REAQRDYLDFLDDEE), identical to the related rat and mouse sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the MCM family.
Protein Information
Name MCM3
Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Cellular Location Nucleus.
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Background

MCM3(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 3), also called RLFB or P1 PROTEIN, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are involved in the initiation of eukaryotic genome replication. The MCM3 gene is mapped to 6p12.2. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

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$ 280.00
Cat# ABO10966
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