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Anti-NDUFA1 Antibody

     
  • WB - Anti-NDUFA1 Antibody ABO10976
    Anti-NDUFA1 antibody, ABO10976, Western blottingLane 1: Rat Cardiac Muscle Tissue Lysate Lane 2: Rat Liver Tissue LysateLane 3: Rat Kidney Tissue LysateLane 4: Rat Brain Tissue LysateLane 5: HT1080 Cell Lysate
    detail
  • IHC - Anti-NDUFA1 Antibody ABO10976
    Anti-NDUFA1 antibody, ABO10976, IHC(P)IHC(P): Rat Cardiac Muscle Tissue
    detail
  • IHC - Anti-NDUFA1 Antibody ABO10976
    Anti-NDUFA1 antibody, ABO10976, IHC(F)IHC(F): Rat Cardiac Muscle Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, ICC
Primary Accession O15239
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1(NDUFA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 4694
Other Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, Complex I-MWFE, CI-MWFE, NADH-ubiquinone oxidoreductase MWFE subunit, NDUFA1
Calculated MW 8072 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.
Tissue Specificity Primarily expressed in heart and skeletal muscle.
Protein Name NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1(54-70aa ISGVDRYYVSKGLENID), different from the related rat and mouse sequences by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name NDUFA1
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein; Matrix side
Tissue Location Primarily expressed in heart and skeletal muscle.
Research Areas
Citations (0)
citation

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Background

NDUFA1(NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX, 1), also called MWFE, B. TAURUS, HOMOLOG OF, encodes a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the NDUFA1 gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity. The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals. The NDUFA1 peptide is one of about 31 components of the hydrophobic protein"(HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function."

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$ 280.00
Cat# ABO10976
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