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Anti-NOX1 Antibody

     
  • WB - Anti-NOX1 Antibody ABO10981
    Anti-NOX1 antibody, ABO10981, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Heart Tissue LysateLane 4: Mouse Heart Tissue Lysate
  •  - Anti-NOX1 Antibody ABO10981
    Anti-NOX1 antibody, ABO10981, HC(P)HC(P): Rat Kidney Tissue
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q9WV87
Host Rabbit
Reactivity Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for NADPH oxidase 1(NOX1) detection. Tested with WB, IHC-P in Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Other Names NADPH oxidase 1, NOX-1, 1.-.-.-, Mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, Nox1, Mox1, Noh1
Calculated MW 65177 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Rat, Mouse, By Heat

Western blot, 0.1-0.5 µg/ml, Mouse, Rat
Subcellular Localization Cell projection, invadopodium membrane ; Multi-pass membrane protein .
Tissue Specificity Expressed in vascular smooth muscle cells.
Protein Name NADPH oxidase 1(NOX-1)
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of rat NOX1(417-431aa WYKFQRAHNKLKTQK), different from the related mouse sequence by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Contains 1 FAD-binding FR-type domain.
Protein Information
Name Nox1
Synonyms Mox1, Noh1
Function Pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism.
Cellular Location Cell projection, invadopodium membrane {ECO:0000250|UniProtKB:Q9Y5S8}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9Y5S8}. Cell membrane {ECO:0000250|UniProtKB:Q9Y5S8}
Tissue Location Expressed in vascular smooth muscle cells. EMBL; AF152963; AAD39542.1; -; mRNA RefSeq; NP_446135.1; NM_053683.1 UniGene; Rn.220465; - ProteinModelPortal; Q9WV87; - SMR; Q9WV87; - STRING; 10116.ENSRNOP00000065995; - ChEMBL; CHEMBL1075231; - PeroxiBase; 5408; RnoNOx01 iPTMnet; Q9WV87; - PhosphoSitePlus; Q9WV87; - PaxDb; Q9WV87; - PRIDE; Q9WV87; - GeneID; 114243; - KEGG; rno:114243; - CTD; 27035; - RGD; 620598; Nox1 eggNOG; KOG0039; Eukaryota eggNOG; ENOG410XNZY; LUCA HOVERGEN; HBG003760; - InParanoid; Q9WV87; - KO; K08008; - PhylomeDB; Q9WV87; - PRO; PR:Q9WV87; - Proteomes; UP000002494; Unplaced GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW GO; GO:0071438; C:invadopodium membrane; IEA:UniProtKB-SubCell GO; GO:0043020; C:NADPH oxidase complex; IDA:RGD GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0016175; F:superoxide-generating NADPH oxidase activity; TAS:RGD GO; GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IMP:RGD GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; IMP:BHF-UCL GO; GO:0000302; P:response to reactive oxygen species; IDA:RGD GO; GO:0007165; P:signal transduction; IDA:RGD GO; GO:0042554; P:superoxide anion generation; IMP:BHF-UCL Gene3D; 3.40.50.80; -; 1 InterPro; IPR000778; Cyt_b245_heavy_chain InterPro; IPR013112; FAD-bd_8 InterPro; IPR017927; Fd_Rdtase_FAD-bd InterPro; IPR013130; Fe3_Rdtase_TM_dom InterPro; IPR013121; Fe_red_NAD-bd_6 InterPro; IPR039261; FNR_nucleotide_bd InterPro; IPR029650; NOX1 InterPro; IPR017938; Riboflavin_synthase-like_b-brl PANTHER; PTHR11972:SF71; PTHR11972:SF71; 1 Pfam; PF08022; FAD_binding_8; 1 Pfam; PF01794; Ferric_reduct; 1 Pfam; PF08030; NAD_binding_6; 1 PRINTS; PR00466; GP91PHOX SUPFAM; SSF52343; SSF52343; 1 SUPFAM; SSF63380; SSF63380; 1 PROSITE; PS51384; FAD_FR; 1 2: Evidence at transcript level; Cell junction; Cell membrane; Cell projection; Complete proteome; Electron transport; FAD; Flavoprotein; Glycoprotein; Heme; Iron; Membrane; Metal-binding; NADP; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix; Transport CHAIN 1 563 NADPH oxidase 1 /FTId=PRO_0000210149 TOPO_DOM 1 8 Cytoplasmic. TRANSMEM 9 31 Helical. TOPO_DOM 32 44 Extracellular. TRANSMEM 45 69 Helical. TOPO_DOM 70 102 Cytoplasmic. TRANSMEM 103 123 Helical. TOPO_DOM 124 167 Extracellular. TRANSMEM 168 188 Helical. TOPO_DOM 189 206 Cytoplasmic. TRANSMEM 207 227 Helical. TOPO_DOM 228 395 Extracellular. TRANSMEM 396 416 Helical. TOPO_DOM 417 563 Cytoplasmic. DOMAIN 54 282 Ferric oxidoreductase DOMAIN 283 390 FAD-binding FR-type {ECO:0000255|PROSITE-ProRule:PRU00716} NP_BIND 337 343 FAD. REGION 396 535 Interaction with NOXO1. METAL 101 101 Iron (heme axial ligand). METAL 115 115 Iron (heme axial ligand). METAL 208 208 Iron (heme axial ligand). METAL 220 220 Iron (heme axial ligand). CARBOHYD 161 161 N-linked (GlcNAc...) asparagine CARBOHYD 241 241 N-linked (GlcNAc...) asparagine SEQUENCE 563 AA; 65177 MW; 2B74BCA021CC3335 CRC64; MGNWLVNHWL SVLFLVSWLG LNIFLFVYVF LNYEKSDKYY YTREILGTAL ALARASALCL NFNSMVILIP VCRNLLSFLR GTCSFCNHTL RKPLDHNLTF HKLVAYMICI FTAIHIIAHL FNFERYSRSQ QAMDGSLASV LSSLFHPEKE DSWLNPIQSP NVTVMYAAFT SIAGLTGVVA TVALVLMVTS AMEFIRRNYF ELFWYTHHLF IIYIICLGIH GLGGIVRGQT EESMSESHPR NCSYSFHEWD KYERSCRSPH FVGQPPESWK WILAPIAFYI FERILRFYRS RQKVVITKVV MHPCKVLELQ MRKRGFTMGI GQYIFVNCPS ISFLEWHPFT LTSAPEEEFF SIHIRAAGDW TENLIRTFEQ QHSPMPRIEV DGPFGTVSED VFQYEVAVLV GAGIGVTPFA SFLKSIWYKF QRAHNKLKTQ KIYFYWICRE TGAFAWFNNL LNSLEQEMDE LGKPDFLNYR LFLTGWDSNI AGHAALNFDR ATDVLTGLKQ KTSFGRPMWD NEFSRIATAH PKSVVGVFLC GPPTLAKSLR KCCRRYSSLD PRKVQFYFNK ETF
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Background

NOX1(NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.

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$ 280.00
Cat# ABO10981
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