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Anti-NOX1 Antibody
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Application 
| WB, IHC-P |
|---|---|
| Primary Accession | Q9WV87 |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for NADPH oxidase 1(NOX1) detection. Tested with WB, IHC-P in Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 114243 |
|---|---|
| Other Names | NADPH oxidase 1, NOX-1, 1.-.-.-, Mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, Nox1, Mox1, Noh1 |
| Calculated MW | 65177 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Rat, Mouse, By Heat Western blot, 0.1-0.5 µg/ml, Mouse, Rat |
| Subcellular Localization | Cell projection, invadopodium membrane ; Multi-pass membrane protein . |
| Tissue Specificity | Expressed in vascular smooth muscle cells. |
| Protein Name | NADPH oxidase 1(NOX-1) |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of rat NOX1(417-431aa WYKFQRAHNKLKTQK), different from the related mouse sequence by one amino acid. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Contains 1 FAD-binding FR-type domain. |
| Name | Nox1 |
|---|---|
| Synonyms | Mox1, Noh1 |
| Function | NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. |
| Cellular Location | Cell projection, invadopodium membrane {ECO:0000250|UniProtKB:Q9Y5S8}; Multi-pass membrane protein. Cell membrane {ECO:0000250|UniProtKB:Q9Y5S8}; Multi-pass membrane protein |
| Tissue Location | Expressed in vascular smooth muscle cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
NOX1(NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.
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