|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Sodium- and chloride-dependent GABA transporter 1(SLC6A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Sodium- and chloride-dependent GABA transporter 1, GAT-1, Solute carrier family 6 member 1, SLC6A1, GABATR, GABT1, GAT1|
|Calculated MW||67074 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Rat, Human, Mouse , By Heat|
Western blot, 0.1-0.5 µg/ml, Mouse, Rat, Human
|Subcellular Localization||Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity). .|
|Protein Name||Sodium- and chloride-dependent GABA transporter 1(GAT-1)|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human GABA Transporter 1(473-487aa WFYGVNRFYDNIQEM), identical to the related rat and mouse sequences.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Synonyms||GABATR, GABT1, GAT1|
|Function||Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane and in a subset of intracellular vesicles Localized at the presynaptic terminals of interneurons (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
GABA transporter 1 (GAT1), also known as sodium- and chloride-dependent GABA transporter 1, is a protein that in humans is encoded by the SLC6A1 gene. GABA Transporter 1 uses Na+ and Cl- to create a gradient, which removes or adds GABA to extracellular spaces in the cerebrum and cerebellum. The stoichiometry for GABA Transporter 1 is 2 Na+: 1 Cl-: 1 GABA. The activity of GAT1 is largely dependent on the presence of Na+, while Cl- assists by increasing the ability for GAT-1 to uptake GABA.
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