Anti-SLC2A5 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IHC-F |
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Primary Accession | Q9WV38 |
Host | Rabbit |
Reactivity | Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 5(SLC2A5) detection. Tested with WB, IHC-P, IHC-F in Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 56485 |
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Other Names | Solute carrier family 2, facilitated glucose transporter member 5, Fructose transporter, Glucose transporter type 5, small intestine, GLUT-5, Slc2a5, Glut5 |
Calculated MW | 55409 MW KDa |
Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, Rat, By Heat Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Rat, Mouse Western blot, 0.1-0.5 µg/ml, Mouse, Rat |
Subcellular Localization | Apical cell membrane ; Multi-pass membrane protein . Membrane ; Multi-pass membrane protein . Localized on the apical membrane of the small intestine and the proximal tubule of the kidney. |
Protein Name | Solute carrier family 2, facilitated glucose transporter member 5 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence in the middle region of mouse SLC2A5 (232-251aa ALQTLRGWKDVHLEMEEIRK), different from the related rat sequence by two amino acids. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | Slc2a5 {ECO:0000312|MGI:MGI:1928369} |
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Function | Functions as a fructose transporter that has only low activity with other monosaccharides (PubMed:12031501, PubMed:19091748). Can mediate the uptake of deoxyglucose, but with low efficiency (By similarity). Essential for fructose uptake in the small intestine (PubMed:19091748, PubMed:26071406). Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose (PubMed:19091748). Required for the development of high blood pressure in response to high dietary fructose intake (PubMed:19091748). |
Cellular Location | Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:P43427}. Note=Localized on the apical membrane of jejunum villi, but also on lateral plasma membranes of the villi (PubMed:18496516, PubMed:26071406). Transport to the cell membrane is dependent on RAB11A (PubMed:26071406). |
Tissue Location | Detected at the apical membrane of villi in the jejunum (PubMed:18496516, PubMed:19091748, PubMed:26071406). Detected in jejunum mucosa (PubMed:26071406). Detected in epididymis and whole testis (at protein level) (PubMed:18417103). Detected in small intestine, kidney and testis (PubMed:12031501, PubMed:18417103, PubMed:19091748). Detected in cochlea, but not in inner or outer cochlear hair cells (PubMed:18417103). |
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Provided below are standard protocols that you may find useful for product applications.
Background
GLUT5(Glucose transporter 5), also known as SLC2A5, is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient. In humans the GLUT5 protein is encoded by the SLC2A5 gene.
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