|Application ||WB, IHC-P, IHC-F, ICC|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 23(SNAP23) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Synaptosomal-associated protein 23, SNAP-23, Vesicle-membrane fusion protein SNAP-23, SNAP23|
|Calculated MW||23354 MW KDa|
|Application Details||Immunocytochemistry , 0.5-1 µg/ml, Human, -|
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
|Subcellular Localization||Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.|
|Tissue Specificity||Ubiquitous. Highest levels where found in placenta.|
|Protein Name||Synaptosomal-associated protein 23(SNAP-23)|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23(192-211aa DTNRDRIDIANARAKKLIDS), different from the related rat and mouse sequences by three amino acids.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the SNAP-25 family.|
|Function||Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.|
|Cellular Location||Cell membrane; Peripheral membrane protein. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome Note=Mainly localized to the plasma membrane|
|Tissue Location||Ubiquitous. Highest levels where found in placenta|
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Provided below are standard protocols that you may find useful for product applications.
SNAP23(Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.
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