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Anti-SPTLC1 Antibody

     
  • WB - Anti-SPTLC1 Antibody ABO11084
    Anti-SPTLC1 antibody, ABO11084, Western blottingAll lanes: Anti SPTLC1 (ABO11084) at 0.5ug/mlLane 1: SW620 Whole Cell Lysate at 40ugLane 2: S549 Whole Cell Lysate at 40ugLane 3: PANC Whole Cell Lysate at 40ugLane 4: U87 Whole Cell Lysate at 40ugPredicted bind size: 53KDObserved bind size: 53KD
    detail
  • IHC - Anti-SPTLC1 Antibody ABO11084
    Anti-SPTLC1 antibody, ABO11084, IHC(P)IHC(P): Human Mammary Cancer Tissue
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession O15269
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Serine palmitoyltransferase 1(SPTLC1) detection. Tested with WB, IHC-P in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 10558
Other Names Serine palmitoyltransferase 1, 2.3.1.50, Long chain base biosynthesis protein 1, LCB 1, Serine-palmitoyl-CoA transferase 1, SPT 1, SPT1, SPTLC1, LCB1
Calculated MW 52744 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat
Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Endoplasmic reticulum membrane ; Single-pass membrane protein .
Tissue Specificity Widely expressed. Not detected in small intestine. .
Protein Name Serine palmitoyltransferase 1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human SPTLC1(138-154aa FYGTFDVHLDLEDRLAK).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
Protein Information
Name SPTLC1
Synonyms LCB1
Function Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core (PubMed:19416851, PubMed:33558762). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed:19416851, PubMed:33558762). The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1- SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA (PubMed:19648650, PubMed:19416851). The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed:19648650, PubMed:19416851, PubMed:33558761, PubMed:33558762). Required for adipocyte cell viability and metabolic homeostasis (By similarity).
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein {ECO:0000250|UniProtKB:O35704}
Tissue Location Widely expressed. Not detected in small intestine.
Research Areas
Citations (0)
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Background

SPTLC1(Serine palmitoyltransferase, long chain base subunit 1), also known as SPT1, LCB1, is a protein which in humans is encoded by the SPTLC1 gene. Dawkins et al.(2001)noted that the SPTLC1 gene maps to chromosome 9q22.1-q22.3. Serine palmitoyltransferase, which consists of two different subunits, is the initial enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.

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$ 280.00
Cat# ABO11084
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