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Anti-Apoptosis Repressor With CARD Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | O60936 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Nucleolar protein 3(NOL3) detection. Tested with WB in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 8996 |
|---|---|
| Other Names | Nucleolar protein 3 {ECO:0000312|HGNC:HGNC:7869}, Apoptosis repressor with CARD, Muscle-enriched cytoplasmic protein, Myp, Nucleolar protein of 30 kDa, Nop30, NOL3 (HGNC:7869) |
| Calculated MW | 22629 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Isoform 1: Nucleus, nucleolus . The SR-rich C-terminus mediates nuclear localization. . |
| Tissue Specificity | Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas. |
| Protein Name | Nucleolar protein 3 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD(91-106aa WDWQHVGPGYRDRSYD), identical to the related rat sequence, and different from the related mouse sequence by one amino acid. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Contains 1 CARD domain. |
| Name | NOL3 (HGNC:7869) |
|---|---|
| Function | [Isoform 1]: May be involved in RNA splicing. |
| Cellular Location | [Isoform 1]: Nucleus, nucleolus. Note=The SR-rich C-terminus mediates nuclear localization. [Isoform 2]: Cytoplasm. Mitochondrion {ECO:0000250|UniProtKB:Q62881}. Sarcoplasmic reticulum {ECO:0000250|UniProtKB:Q62881}. Membrane; Lipid-anchor. Note=Phosphorylation at Thr-149 results in translocation to mitochondria. Colocalized with mitochondria in response to oxidative stress. {ECO:0000250|UniProtKB:Q62881} |
| Tissue Location | Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas |
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Provided below are standard protocols that you may find useful for product applications.
Background
NOL3(Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al.(1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.
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