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Anti-MPG Antibody

     
  • WB - Anti-MPG Antibody ABO11133
    Anti- MPG antibody, ABO11133, Western blottingAll lanes: Anti MPG (ABO11133) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P29372
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for DNA-3-methyladenine glycosylase(MPG) detection. Tested with WB in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 4350
Other Names DNA-3-methyladenine glycosylase, 3.2.2.21, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase, MPG, AAG, ANPG, MID1
Calculated MW 32869 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Cytoplasm . Mitochondrion matrix, mitochondrion nucleoid . Nucleus .
Protein Name DNA-3-methyladenine glycosylase
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human MPG(228-243aa NKSFDQRDLAQDEAVW), different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the DNA glycosylase MPG family.
Protein Information
Name MPG
Synonyms AAG, ANPG, MID1
Function Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Cellular Location Cytoplasm. Mitochondrion matrix, mitochondrion nucleoid. Nucleus
Citations (0)

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Background

MPG(N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The MPG gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo, Hang et al.(1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine(meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

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$ 240.00
Cat# ABO11133
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