Anti-MPG Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P29372 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for DNA-3-methyladenine glycosylase(MPG) detection. Tested with WB in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 4350 |
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Other Names | DNA-3-methyladenine glycosylase, 3.2.2.21, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, ADPG, N-methylpurine-DNA glycosylase, MPG, AAG, ANPG, MID1 |
Calculated MW | 32869 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
Subcellular Localization | Cytoplasm . Mitochondrion matrix, mitochondrion nucleoid . Nucleus . |
Protein Name | DNA-3-methyladenine glycosylase |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MPG(228-243aa NKSFDQRDLAQDEAVW), different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the DNA glycosylase MPG family. |
Name | MPG |
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Synonyms | AAG, ANPG, MID1 |
Function | Hydrolysis of the deoxyribose N-glycosidic bond to excise 3- methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. |
Cellular Location | Cytoplasm. Mitochondrion matrix, mitochondrion nucleoid. Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
Background
MPG(N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The MPG gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo, Hang et al.(1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine(meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.
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