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Anti-Gli2 Antibody
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- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P10070 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Zinc finger protein GLI2(GLI2) detection. Tested with WB in Human. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 2736 |
|---|---|
| Other Names | Zinc finger protein GLI2, GLI family zinc finger protein 2 {ECO:0000312|HGNC:HGNC:4318}, Tax helper protein, GLI2 (HGNC:4318) |
| Calculated MW | 167783 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human |
| Subcellular Localization | Nucleus . Cytoplasm . In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus. . |
| Tissue Specificity | Isoform 1 and isoform 4 are expressed in HTLV- 1-infected T-cell lines (at protein level). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines. . |
| Protein Name | Zinc finger protein GLI2 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2(1444-1463aa MLYYYGQIHMYEQDGGLENL). |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the GLI C2H2-type zinc-finger protein family. |
| Name | GLI2 (HGNC:4318) |
|---|---|
| Function | Functions as a transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992, PubMed:26565916). Functions as a transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856). |
| Cellular Location | Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q0VGT2}. Cell projection, cilium {ECO:0000250|UniProtKB:Q0VGT2}. Note=STK36 promotes translocation to the nucleus. In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus {ECO:0000250|UniProtKB:Q0VGT2} [Isoform 2]: Nucleus |
| Tissue Location | Expressed in breast cancers (at protein level) (PubMed:26565916). Isoform 1 and isoform 4 are expressed in HTLV-1- infected T-cell lines (at protein level) (PubMed:9557682). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines (PubMed:9557682). Isoform 3 and isoform 4 are weakly expressed in HTLV- 1-infected T-cell lines (PubMed:9557682). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
GLI2(Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel(Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes; thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al.(1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al.(2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.
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