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Anti-DDX6 Antibody

     
  • WB - Anti-DDX6 Antibody ABO11272
    Anti-DDX6 antibody, ABO11272, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant human DDX6, 41.8KD(162aa tag+S330-P483)Lane 1: Recombinant Human DDX6 Protein 10ngLane 2: Recombinant Human DDX6 Protein 5ngLane 3: Recombinant Human DDX6 Protein 2.5ng Lane 4: Recombinant Human DDX6 Protein 1.25ng
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P26196
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX6(DDX6) detection. Tested with WB in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 1656
Other Names Probable ATP-dependent RNA helicase DDX6, 3.6.4.13, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK, DDX6, HLR2, RCK
Calculated MW 54417 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cytoplasm, P-body .
Tissue Specificity Abundantly expressed in most tissues.
Protein Name Probable ATP-dependent RNA helicase DDX6
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human DDX6(440-453aa YDDRFNLKSIEEQL), identical to the related rat and mouse sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.
Protein Information
Name DDX6
Synonyms HLR2, RCK
Function Essential for the formation of P-bodies, cytosolic membrane- less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed:25995375, PubMed:27342281, PubMed:31422817). Plays a role in P- bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).
Cellular Location Cytoplasm, P-body. Cytoplasm. Nucleus. Note=Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:28216671). Upon cellular stress, relocalizes to stress granules (PubMed:26184334).
Tissue Location Abundantly expressed in most tissues.
Citations (0)
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Background

DDX6(DEAD/H BOX 6), also known as HLR2 or p54, is an enzyme that in humans is encoded by the DDX6 gene. DDX6 belongs to the DEAD box family of putative RNA helicases that contain a characteristic asp-glu-ala-asp(DEAD) box motif(Seto et al., 1995). Tunnacliffe et al.(1993) assigned the DDX6 gene more precisely using a panel of sequence tagged sites(STSs) representing 30 markers previously assigned to 11q23. Using mass spectroscopy, Fenger-Gron et al.(2005) found that RCK, EDC3(YJDC), and HEDLS(RCD8) coimmunopurified with DCP1A and DCP2 from HEK293 cell lysates. Overexpression of DCP2, RCK, or EDC3 in HeLa cells reduced the association of endogenous DCP1A and XRN1 with cytoplasmic P bodies.

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$ 240.00
Cat# ABO11272
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