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Anti-Hamartin Antibody

     
  • WB - Anti-Hamartin Antibody ABO11319
    Anti-Hamartin antibody, ABO11319, Western blottingAll lanes: Anti Hamartin (ABO11319) at 0.5ug/mlLane 1: Rat Skeletal Muscle Tissue Lysate at 50ugLane 2: Rat Heart Tissue Lysate at 50ugLane 3: Rat Brain Tissue Lysate at 50ugLane 4: Rat Lung Tissue Lysate at 50ugLane 5: 293T Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: HT1080 Whole Cell Lysate at 40ugLane 8: SMMC Whole Cell Lysate at 40ugPredicted bind size: 130KDObserved bind size: 160KD
  • IHC - Anti-Hamartin Antibody ABO11319
    Anti-Hamartin antibody, ABO11319, IHC(P)IHC(P): Human Mammary Cancer Tissue
  • IHC - Anti-Hamartin Antibody ABO11319
    Anti-Hamartin antibody, ABO11319, IHC(P)IHC(P): Rat Brain Tissue
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q92574
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Hamartin(TSC1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 7248
Other Names Hamartin, Tuberous sclerosis 1 protein, TSC1, KIAA0243, TSC
Calculated MW 129767 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Cytoplasm . Membrane ; Peripheral membrane protein . At steady state found in association with membranes.
Tissue Specificity Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
Protein Name Hamartin
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin(1150-1164aa QLHIMDYNETHHEHS), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name TSC1
Synonyms KIAA0243, TSC
Function In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400). Seems not to be required for TSC2 GAP activity towards RHEB (PubMed:15340059). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (PubMed:29127155). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (PubMed:29127155). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (PubMed:16464865, PubMed:29127155).
Cellular Location Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes.
Tissue Location Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells
Research Areas
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Background

TSC1(Tuberous Sclerosis 1), also called HAMARTIN or TSC, is a human protein and gene. As part of a comprehensive strategy to identify the gene mutant in tuberous sclerosis-1, van Slegtenhorst et al.(1997) developed an overlapping contig of clones from the 1.4-Mb TSC1 region on chromosome 9. Benvenuto et al.(2000) showed that overexpression of the TSC1 gene in rat fibroblasts inhibits growth and causes changes in cell morphology. Van Slegtenhorst et al.(1998) showed that hamartin and tuberin associate physically in vivo, however, and that the interaction is mediated by predicted coiled-coil domains.

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$ 280.00
Cat# ABO11319
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