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Anti-IKK Beta Antibody

     
  • WB - Anti-IKK Beta Antibody ABO11343
    Anti-IKK beta antibody, ABO11343, Western blottingAll lanes: Anti IKK beta (ABO11343) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: HT1080 Whole Cell Lysate at 40ugLane 4: SKOV Whole Cell Lysate at 40ugLane 5: M231 Whole Cell Lysate at 40ugLane 6: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 87KDObserved bind size: 87KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O14920
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Inhibitor of nuclear factor kappa-B kinase subunit beta(IKBKB) detection. Tested with WB in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 3551
Other Names Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, 2.7.11.10, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB
Calculated MW 86564 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Cytoplasm. Nucleus. Membrane raft. Colocalized with DPP4 in membrane rafts.
Tissue Specificity Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Protein Name Inhibitor of nuclear factor kappa-B kinase subunit beta
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human IKK beta(735-750aa TALDWSWLQTEEEEHS).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Protein Information
Name IKBKB
Synonyms IKKB
Function Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF- dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
Cellular Location Cytoplasm. Nucleus. Membrane raft. Note=Colocalized with DPP4 in membrane rafts.
Tissue Location Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood
Research Areas
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Background

IKBKB(Inhibitor of Kappa Light Chain Gene Enhancer in B Cells, Kinase of, Beta), also known as IKKB or NFKBIKB , is a protein that in humans is encoded by the IKBKB gene. Shindo et al.(1998) mapped the IKBKB gene to chromosome 8p12-p11 by FISH. Mercurio et al.(1997) found that mutations in IKK2 had a more pronounced effect upon NFKB activation than did comparable mutations in IKK1. Yin et al.(1998) tested the activity of various antiinflammatory agents on the IKK complex. They demonstrated that aspirin and sodium salicylate specifically inhibit IKK-beta activity in vitro and in vivo by binding to IKK-beta to reduce ATP binding.

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$ 240.00
Cat# ABO11343
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