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Anti-PADI4/PAD4 Antibody

     
  • WB - Anti-PADI4/PAD4 Antibody ABO11351
    Anti-PADI4/PAD4 antibody, ABO11351, Western blottingLane 1: PANC Cell LysateLane 2: 293T Cell Lysate
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9UM07
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Protein-arginine deiminase type-4(PADI4) detection. Tested with WB in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 23569
Other Names Protein-arginine deiminase type-4, 3.5.3.15, HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV, PADI4, PAD4, PADI5, PDI5
Calculated MW 74079 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
Tissue Specificity Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. .
Protein Name Protein-arginine deiminase type-4
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human PADI4(492-512aa ASPRSCYKLFQEQQNEGHGEA).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name PADI4
Synonyms PAD4, PADI5, PDI5
Function Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg- 8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed:18209087). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed:15345777). Citrullinates EP300/P300 at 'Arg- 2142', which favors its interaction with NCOA2/GRIP1 (PubMed:15731352).
Cellular Location Cytoplasm. Nucleus. Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils and neutrophils.
Tissue Location Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes
Research Areas
Citations (0)
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Background

PADI4(Peptidylarginine Deiminase, Type IV), also known as PAD or PAD4, is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases, such as PADI4, make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al., 1999). Suzuki et al.(2003) found from sequence data that the PADI4 gene maps to 1p36. Cuthbert et al.(2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that PADI4 specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PADI4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PADI4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that PADI4 could repress hormone receptor-mediated gene induction. PADI4 was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role for PADI4.

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$ 240.00
Cat# ABO11351
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