Anti-Prothrombin Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P18292 |
Host | Rabbit |
Reactivity | Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Prothrombin(F2) detection. Tested with WB in Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Other Names | Prothrombin, 3.4.21.5, Coagulation factor II, Activation peptide fragment 1, Activation peptide fragment 2, Thrombin light chain, Thrombin heavy chain, F2 |
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Calculated MW | 70412 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Rat |
Protein Name | Prothrombin |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of rat Prothrombin(600-612aa THVFRLKRWMQKV). |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the peptidase S1 family. |
Name | F2 |
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Function | Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. |
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Provided below are standard protocols that you may find useful for product applications.
Background
F2(Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. Royle et al.(1987) assigned the gene for human prothrombin(F2) to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII(F8), and XIII(F13A1). Thrombin also inhibits coagulation by activating protein C.
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