|Description||Rabbit IgG polyclonal antibody for Coagulation factor VIII(F8) detection. Tested with WB in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Coagulation factor VIII, Antihemophilic factor, AHF, Procoagulant component, Factor VIIIa heavy chain, 200 kDa isoform, Factor VIIIa heavy chain, 92 kDa isoform, Factor VIII B chain, Factor VIIIa light chain, F8, F8C|
|Calculated MW||267009 MW KDa|
|Application Details||Western blot, 0.1-0.5 µg/ml, Human|
|Subcellular Localization||Secreted, extracellular space.|
|Protein Name||Coagulation factor VIII|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Factor VIII(1264-1278aa APVLQDFRSLNDSTN).|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Sequence Similarities||Belongs to the multicopper oxidase family.|
|Function||Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.|
|Cellular Location||Secreted, extracellular space.|
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Provided below are standard protocols that you may find useful for product applications.
Coagulation Factor VIII(FVIII) is an essential blood-clotting protein, also known as anti-hemophilic factor(AHF). By in situ hybridization, Tantravahi et al.(1986) concluded that the F8 gene is located in the proximal part of chromosome Xq28 with probes DX13 and St14 distally located. The F8 gene encodes coagulation factor VIII, a large plasma glycoprotein that functions in the blood coagulation cascade as a cofactor for the factor IXa-dependent activation of factor X(F10). Factor VIII is activated proteolytically by a variety of coagulation enzymes, including thrombin(F2). Factor VIII is tightly associated in the blood with von Willebrand factor(VWF), which serves as a protective carrier protein for factor VIII(Toole et al., 1984; Hoyer, 1994).
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