Anti-SLC2A5 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | P22732 |
Host | Rabbit |
Reactivity | Human |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 5(SLC2A5) detection. Tested with WB in Human. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 6518 |
---|---|
Other Names | Solute carrier family 2, facilitated glucose transporter member 5, Fructose transporter, Glucose transporter type 5, small intestine, GLUT-5, SLC2A5, GLUT5 |
Calculated MW | 54974 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Human |
Subcellular Localization | Apical cell membrane ; Multi- pass membrane protein . Membrane; Multi-pass membrane protein. Localized on the apical membrane of the small intestine and the proximal tubule of the kidney. . |
Tissue Specificity | Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue. |
Protein Name | Solute carrier family 2, facilitated glucose transporter member 5 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SLC2A5 (481-501aa VSEVYPEKEELKELPPVTSEQ). |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. |
Name | SLC2A5 (HGNC:11010) |
---|---|
Function | Functions as a fructose transporter that has only low activity with other monosaccharides (PubMed:8333543, PubMed:16186102, PubMed:28083649, PubMed:17710649, PubMed:29548810). Can mediate the uptake of 2-deoxyglucose, but with low efficiency (PubMed:1695905). Essential for fructose uptake in the small intestine (By similarity). Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose (By similarity). Required for the development of high blood pressure in response to high dietary fructose intake (By similarity). |
Cellular Location | Apical cell membrane {ECO:0000250|UniProtKB:Q9WV38}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9WV38}. Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9WV38}. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:P43427}. Note=Localized on the apical membrane of jejunum villi, but also on lateral plasma membranes of the villi. Transport to the cell membrane is dependent on RAB11A {ECO:0000250|UniProtKB:Q9WV38} |
Tissue Location | Detected in skeletal muscle, and in jejunum brush border membrane and basolateral membrane (at protein level) (PubMed:7619085). Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
SLC2A5, also known as GLUT5(Glucose transporter 5), is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient. In humans the GLUT5 protein is encoded by the SLC2A5 gene.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.