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Anti-Smad1 Antibody

     
  • WB - Anti-Smad1 Antibody ABO11421
    Anti-Smad1 antibody, ABO11421, Western blottingLane 1: SMMC Cell LysateLane 2: K562 Cell LysateLane 3: HT1080 Cell LysateLane 4: HELA Cell LysateLane 5: JURKAT Cell Lysate
    detail
  • IHC - Anti-Smad1 Antibody ABO11421
    Anti-Smad1 antibody, ABO11421, IHC(P)IHC(P): Human Lung Cancer Tissue
    detail
  • ICC - Anti-Smad1 Antibody ABO11421
    Anti-Smad1 antibody, ABO11421, ICCICC: A549 Cell
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession P70340
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 1(SMAD1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 17125
Other Names Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD family member 1, SMAD 1, Smad1, Smad1, Madh1, Madr1
Calculated MW 52157 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cytoplasm . Nucleus . In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4. Colocalizes with LEMD3 at the nucleus inner membrane (By similarity). .
Tissue Specificity Ubiquitous.
Protein Name Mothers against decapentaplegic homolog 1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse Smad1(253-267aa AEISRGDVQAVAYEE), different from the related rat sequence by one amino acid, and different from the related human sequence by two amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the dwarfin/SMAD family.
Protein Information
Name Smad1
Synonyms Madh1, Madr1
Function Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed:11566864, PubMed:15329343, PubMed:21420501, PubMed:35594155). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 (By similarity). Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7- mediated nuclear import (PubMed:34995814).
Cellular Location Cytoplasm. Nucleus Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane (By similarity). Exported from the nucleus to the cytoplasm when dephosphorylated PubMed:25755279. {ECO:0000250|UniProtKB:Q15797, ECO:0000269|PubMed:25755279}
Tissue Location Ubiquitous.
Research Areas
Citations (0)
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Background

Mothers against decapentaplegic homolog 1 also known as SMAD family member 1 or SMAD1 is a protein that in humans is encoded by the SMAD1 gene. SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic"(Mad) and the C. elegans gene Sma. The gene was assigned to human chromosome 4q31.21. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways."

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$ 280.00
Cat# ABO11421
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