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Anti-SMAD5 Antibody

     
  • IHC - Anti-SMAD5 Antibody ABO11422
    Anti-SMAD5 antibody, ABO11422, IHC(P)IHC(P): Rat Intestine Tissue
  • IHC - Anti-SMAD5 Antibody ABO11422
    Anti-SMAD5 antibody, ABO11422, IHC(P)IHC(P): Human Intestinal Cancer Tissue
  • IHC - Anti-SMAD5 Antibody ABO11422
    Anti-SMAD5 antibody, ABO11422, IHC(P)IHC(P): Human Lung Cancer Tissue
  • WB - Anti-SMAD5 Antibody ABO11422
    Anti-SMAD5 antibody, ABO11422, Western blottingAll lanes: Anti SMAD5 (ABO11422) at 0.5ug/mlLane 1: K562 Whole Cell Lysate at 40ugLane 2: JURKAT Whole Cell Lysate at 40ugLane 3: PC 1-2 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugPredicted bind size: 52 KDObserved bind size: 52KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q99717
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 5(SMAD5) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 4090
Other Names Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5
Calculated MW 52258 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Tissue Specificity Ubiquitous.
Protein Name Mothers against decapentaplegic homolog 5
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human SMAD5(248-267aa IPQIMPSISSRDVQPVAYEE), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the dwarfin/SMAD family.
Protein Information
Name SMAD5
Synonyms MADH5
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Background

Mother against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins(BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.

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$ 280.00
Cat# ABO11422
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