|Application ||WB, IHC-P, ICC|
|Description||Rabbit IgG polyclonal antibody for Linker for activation of T-cells family member 1(LAT) detection. Tested with WB, IHC-P, ICC in Human.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein, pp36, p36-38, LAT|
|Calculated MW||27930 MW KDa|
|Application Details||Immunocytochemistry , 0.5-1 µg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat
Western blot, 0.1-0.5 µg/ml, Human
|Subcellular Localization||Cell membrane ; Single-pass type III membrane protein . Present in lipid rafts.|
|Tissue Specificity||Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level). .|
|Protein Name||Linker for activation of T-cells family member 1|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human LAT(242-262aa QEAEEVEEEGAPDYENLQELN), different from the related mouse and rat sequences by six amino acids.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.|
|Cellular Location||Cell membrane; Single-pass type III membrane protein Note=Present in lipid rafts|
|Tissue Location||Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Linker for activation of T cells, also known as LAT, is a protein which in humans is encoded by the LAT gene. This gene is mapped to 16p11.2. The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor(TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement.
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