|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit IgG polyclonal antibody for Neutral amino acid transporter A(SLC1A4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Other Names||Neutral amino acid transporter A, Alanine/serine/cysteine/threonine transporter 1, ASCT-1, SATT, Solute carrier family 1 member 4, SLC1A4, ASCT1, SATT|
|Calculated MW||55723 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
|Subcellular Localization||Membrane ; Multi-pass membrane protein . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Tissue Specificity||Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined.|
|Protein Name||Neutral amino acid transporter A|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SLC1A4(495-512aa EAIPNCKSEEETSPLVTH), different from the related rat and mouse sequences by one amino acid.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Function||Transporter for alanine, serine, cysteine, and threonine. Exhibits sodium dependence.|
|Cellular Location||Membrane; Multi-pass membrane protein Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
|Tissue Location||Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined|
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Provided below are standard protocols that you may find useful for product applications.
Neutral amino acid transporter A, also called SLC1A4 or ASCT1, is a protein that in humans is encodes by the SLC1A4 gene. The SLC1A4 gene is mapped to chromosome 2p14 based on an alignment of the SLC1A4 sequence with the genomic sequence(GRCh37). ASCT1 does not transport glutamate or aspartate but alanine, serine, cysteine, and threonine instead. It is found that the ASCT1 transporter functions primarily as an amino acid exchanger. Transport is associated with a chloride channel activity that is thermodynamically uncoupled from animo acid transport. This gene also exhibits sodium dependence.
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