Anti-SLC22A7 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q9Y694 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Solute carrier family 22 member 7(SLC22A7) detection. Tested with WB in Human;Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 10864 |
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Other Names | Solute carrier family 22 member 7, Novel liver transporter, Organic anion transporter 2, hOAT2, SLC22A7, NLT, OAT2 |
Calculated MW | 60026 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
Subcellular Localization | Basolateral cell membrane ; Multi- pass membrane protein . Apical side of the renal tubule. |
Tissue Specificity | Expressed in liver and kidney. . |
Protein Name | Solute carrier family 22 member 7 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SLC22A7(514-530aa ETRQAQLPETIQDVERK), different from the related rat and mouse sequences by two amino acids. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. |
Name | SLC22A7 (HGNC:10971) |
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Synonyms | NLT, OAT2 |
Function | [Isoform 2]: Functions as a Na(+)-independent bidirectional multispecific transporter (PubMed:11327718, PubMed:18216183, PubMed:21446918, PubMed:28945155). Contributes to the renal and hepatic elimination of endogenous organic compounds from the systemic circulation into the urine and bile, respectively (PubMed:11327718, PubMed:25904762). Capable of transporting a wide range of purine and pyrimidine nucleobases, nucleosides and nucleotides, with cGMP, 2'deoxyguanosine and GMP being the preferred substrates (PubMed:11327718, PubMed:18216183, PubMed:26377792, PubMed:28945155). Functions as a pH- and chloride-independent cGMP bidirectional facilitative transporter that can regulate both intracellular and extracellular levels of cGMP and may be involved in cGMP signaling pathways (PubMed:18216183, PubMed:26377792). Mediates orotate/glutamate bidirectional exchange and most likely display a physiological role in hepatic release of glutamate into the blood (PubMed:21446918). Involved in renal secretion and possible reabsorption of creatinine (PubMed:25904762, PubMed:28945155). Able to uptake prostaglandin E2 (PGE2) and may contribute to PGE2 renal excretion (Probable). Also transports alpha-ketoglutarate and urate (PubMed:11327718, PubMed:26377792). Apart from the orotate/glutamate exchange, the counterions for the uptake of other SLC22A7/OAT2 substrates remain to be identified (PubMed:26377792). |
Cellular Location | [Isoform 2]: Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Localized to the basolateral side of the proximal tubules (PubMed:12023506, PubMed:25904762). Apical side of the renal tubule (PubMed:25904762). |
Tissue Location | Mainly expressed in liver and kidney (PubMed:11327718, PubMed:18216183, PubMed:21446918, PubMed:25904762) In kidney, expressed in proximal tubular cells (PubMed:25904762). Also expressed in pancreas, small intestine, spinal cord, lung, brain and heart (PubMed:18216183). Expressed in fetal liver (PubMed:21446918) |
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Background
Solute carrier family 22 member 7, also called SLC22A7 or OAT2 is a protein that in humans is encoded by the SLC22A7 gene. This gene is mapped to 6p21.1. The protein encoded by this gene is involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. This gene transports prostaglandin E2, prostaglandin F2, tetracycline, bumetanide, estrone sulfate, glutarate, dehydroepiandrosterone sulfate, allopurinol, 5-fluorouracil, paclitaxel, L-ascorbic acid, salicylate, ethotrexate, and alpha-ketoglutarate.
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