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Anti-Rab9 Antibody

     
  • WB - Anti-Rab9 Antibody ABO11590
    Anti-Rab9 antibody, ABO11590, All Western blottingAll lanes: Anti-RAB9A(ABO11590) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 40ugLane 2: Mouse Brain Tissue Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: PC12 Whole Cell Lysate at 40ugLane 5: NIH Whole Cell Lysate at 40ugLane 6: A431 Whole Cell Lysate at 40ugLane 7: 293T Whole Cell Lysate at 40ugPredicted bind size: 23KDObserved bind size: 23KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P51151
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Ras-related protein Rab-9A(RAB9A) detection. Tested with WB in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 9367
Other Names Ras-related protein Rab-9A, RAB9A, RAB9
Calculated MW 22838 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cell membrane ; Lipid-anchor ; Cytoplasmic side . Endoplasmic reticulum membrane . Golgi apparatus membrane . Late endosome. Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasmic vesicle, phagosome. Colocalizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or M.tuberculosis.
Protein Name Ras-related protein Rab-9A
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9(178-193aa RSDHLIQTDTVNLHRK), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name RAB9A
Synonyms RAB9
Function Involved in the transport of proteins between the endosomes and the trans Golgi network. Involved in the recruitment of SGSM2 to melanosomes and is required for the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome Cytoplasmic vesicle, phagosome membrane; Lipid- anchor; Cytoplasmic side. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q9R0M6}. Melanosome {ECO:0000250|UniProtKB:Q9R0M6}. Note=Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211)
Research Areas
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Background

Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is directly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What's more, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.

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$ 240.00
Cat# ABO11590
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