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Anti-SP100 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P23497 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Nuclear autoantigen Sp-100(SP100) detection. Tested with WB in Human. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 6672 |
|---|---|
| Other Names | Nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, Speckled 100 kDa, SP100 |
| Calculated MW | 100417 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human |
| Subcellular Localization | Nucleus. Nucleus, PML body. Cytoplasm. Differences in the subnuclear localization of the different isoforms seem to exist and may also be cell cycle- and interferon- dependent. Accumulates in the cytoplasm upon FAS activation. |
| Tissue Specificity | Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines. |
| Protein Name | Nuclear autoantigen Sp-100 |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SP100(664-677aa YTLKVLMENKFLPE). |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Contains 2 HMG box DNA-binding domains. |
| Name | SP100 |
|---|---|
| Function | Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to PubMed:11909962. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to PubMed:15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation. |
| Cellular Location | Nucleus. Nucleus, PML body. Nucleus, nuclear body. Cytoplasm Note=Differences in the subnuclear localization of the different isoforms seem to exist and may also be cell cycle- and interferon- dependent. Accumulates in the cytoplasm upon FAS activation |
| Tissue Location | Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines |
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Provided below are standard protocols that you may find useful for product applications.
Background
SP100, also known as LYSP100B, INCLUDED, encodes a subnuclear organelle and major component of the PML(promyelocytic leukemia)-SP100 nuclear bodies. SP100 and PML are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is though to play a role in tumorigenesis, immunity, and gene regulation. It has been found that HP1 expression is enhanced when SP100 synthesis is induced by interferon. SP100 can function as a transcriptional coactivator of ETS1 and ETS2, and under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA.
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