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Anti-ACSL1 Picoband Antibody

     
  • WB - Anti-ACSL1 Picoband Antibody ABO11643
    Western blot analysis of ACSL1 expression in rat liver extract (lane 1) HEPA whole cell lysates (lane 2) and A549 whole cell lysates (lane 3). ACSL1 at 78KD was detected using rabbit anti- ACSL1 Antigen Affinity purified polyclonal antibody (Catalog # ABO11643) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
    detail
  • IHC - Anti-ACSL1 Picoband Antibody ABO11643
    ACSL1 was detected in paraffin-embedded sections of mouse kidney tissues using rabbit anti- ACSL1 Antigen Affinity purified polyclonal antibody (Catalog # ABO11643) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-ACSL1 Picoband Antibody ABO11643
    ACSL1 was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- ACSL1 Antigen Affinity purified polyclonal antibody (Catalog # ABO11643) at 1 ??g/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-ACSL1 Picoband Antibody ABO11643
    ACSL1 was detected in paraffin-embedded sections of human liver cancer tissues using rabbit anti- ACSL1 Antigen Affinity purified polyclonal antibody (Catalog # ABO11643) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P33121
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Long-chain-fatty-acid--CoA ligase 1(ACSL1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 2180
Other Names Long-chain-fatty-acid--CoA ligase 1, 6.2.1.3, Acyl-CoA synthetase 1, ACS1, Long-chain acyl-CoA synthetase 1, LACS 1, Long-chain acyl-CoA synthetase 2, LACS 2, Long-chain fatty acid-CoA ligase 2, Palmitoyl-CoA ligase 1, Palmitoyl-CoA ligase 2, ACSL1, FACL1, FACL2, LACS, LACS1, LACS2
Calculated MW 77943 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Mitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein . Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .
Tissue Specificity Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas. .
Protein Name Long-chain-fatty-acid--CoA ligase 1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human ACSL1 recombinant protein (Position: D604-V698). Human ACSL1 shares 81.1% and 86.3% amino acid (aa) sequence identity with mouse and rat ACSL1, respectively.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name ACSL1 (HGNC:3569)
Function Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:24269233, PubMed:22633490, PubMed:21242590). Preferentially uses palmitoleate, oleate and linoleate (PubMed:24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).
Cellular Location Mitochondrion outer membrane; Single-pass type III membrane protein. Peroxisome membrane; Single-pass type III membrane protein. Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein
Tissue Location Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
Research Areas
Citations (0)
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Background

Long-chain-fatty-acid—CoA ligase 1 is an enzyme that in humans is encoded by the ACSL1 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. This specific protein is most commonly found in mitochondria and peroxisomes.

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$ 280.00
Cat# ABO11643
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