|Application ||WB, IHC-P|
|Description||Rabbit IgG polyclonal antibody for 30 kDa adipocyte complement-related protein(ADIPOQ) detection. Tested with WB, IHC-P in Mouse;Rat.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Calculated MW||26410 MW KDa|
|Application Details||Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, Rat, By Heat|
Western blot, 0.1-0.5 µg/ml, Rat
|Protein Name||30 kDa adipocyte complement-related protein|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived rat Adiponectin recombinant protein (Position: I19-N244). Rat Adiponectin shares 90% amino acid (aa) sequence identity with mouse Adiponectin.|
|Purification||Immunogen affinity purified.|
|Cross Reactivity||No cross reactivity with other proteins|
|Storage||At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
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Provided below are standard protocols that you may find useful for product applications.
Adiponectin, also known as APM1 or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. It is mapped to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, it has been showed that Adiponectin is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, it has been found that links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. This gene is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.
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