Anti-CCL4/MIP-1 beta Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P50230 |
Host | Rabbit |
Reactivity | Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for C-C motif chemokine 4(CCL4) detection. Tested with WB in Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 116637 |
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Other Names | C-C motif chemokine 4, Macrophage inflammatory protein 1-beta, MIP-1-beta, Small-inducible cytokine A4, Ccl4, Mip1b, Scya4 |
Calculated MW | 10234 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Rat |
Subcellular Localization | Secreted. |
Protein Name | C-C motif chemokine 4 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen | E.coli-derived rat CCL4 recombinant protein (Position: A24-N92). Rat CCL4 shares 80% and 86% amino acid (aa) sequences identity with human and mouse CCL4, respectively. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | Ccl4 |
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Synonyms | Mip1b, Scya4 |
Function | Monokine with inflammatory and chemokinetic properties. |
Cellular Location | Secreted. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in human is encoded by the CCL4 gene. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize CCL4. Based on functional analysis of CCL4, one or more additional beta chemokine receptors that transduce CCL4 signals must also exist. MIP-1alpha and CCL4 have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines(11, 14).
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