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Anti-CD3 Epsilon Picoband Antibody

     
  • WB - Anti-CD3 Epsilon Picoband Antibody ABO11801
    Anti-CD3 epsilon Picoband antibody, ABO11801-1.jpgAll lanes: Anti CD3 Epsilon (ABO11801) at 0.5ug/mlWB : Recombinant Human CD3epsilon Protein 0.5ng Predicted bind size: 40KDObserved bind size: 40KD
  •  - Anti-CD3 Epsilon Picoband Antibody ABO11801
    Anti-CD3 epsilon Picoband antibody, ABO11801-2.jpgAll lanes: Anti CD3 Epsilon (ABO11801) at 0.5ug/mlLane 1: JURKAT Whole Cell Lysate at 40ugLane 2: CEM Whole Cell Lysate at 40ugPredicted bind size: 23KDObserved bind size: 23KD
  • IHC - Anti-CD3 Epsilon Picoband Antibody ABO11801
    Anti-CD3 epsilon Picoband antibody, ABO11801-3.JPGIHC(P): Human Tonsil Tissue
  • IHC - Anti-CD3 Epsilon Picoband Antibody ABO11801
    Anti-CD3 epsilon Picoband antibody, ABO11801-4.JPGIHC(P): Mouse Spleen Tissue
  • IHC - Anti-CD3 Epsilon Picoband Antibody ABO11801
    Anti-CD3 epsilon Picoband antibody, ABO11801-5.JPGIHC(P): Rat Spleen Tissue
  • IHC - Anti-CD3 Epsilon Picoband Antibody ABO11801
    Anti-CD3 epsilon Picoband antibody, pb9093-6.JPGIHC(F): Rat Spleen Tissue
  • IHC - Anti-CD3 Epsilon Picoband Antibody ABO11801
    Anti-CD3 epsilon Picoband antibody, pb9093-7.JPGIHC(F): Mouse Spleen Tissue
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IHC-P, IHC-F, IF, IC, ICC
Primary Accession P07766
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 916
Other Names T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Calculated MW 23147 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-1 µg/ml, Mouse, Rat, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Membrane; Single-pass type I membrane protein.
Protein Name T-cell surface glycoprotein CD3 epsilon chain
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Contains 1 Ig-like (immunoglobulin-like) domain.
Protein Information
Name CD3E
Synonyms T3E
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down- regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
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Background

CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

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$ 280.00
Cat# ABO11801
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