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Anti-Gli1 Picoband Antibody

     
  • WB - Anti-Gli1 Picoband Antibody ABO11814
    Anti-Gli1 Picoband antibody, ABO11814-1.jpgAll lanes: Anti GLI1 (ABO11814) at 0.5ug/mlWB: Recombinant Human GLI1 Protein 0.5ngPredicted bind size: 47KDObserved bind size: 47KD
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  •  - Anti-Gli1 Picoband Antibody ABO11814
    Anti-Gli1 Picoband antibody, ABO11814-2.jpgAll lanes: Anti GLI1 (ABO11814) at 0.5ug/mlLane 1: U87 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: SKOV Whole Cell Lysate at 40ugLane 5: HT1080 Whole Cell Lysate at 40ugLane 6: COLO320 Whole Cell Lysate at 40ugLane 7: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 118KDObserved bind size: 118KD
    detail
  • IHC - Anti-Gli1 Picoband Antibody ABO11814
    Anti-Gli1 Picoband antibody, ABO11814-3.JPGIHC(P): Human Intestinal Cancer Tissue
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P08151
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Zinc finger protein GLI1(GLI1) detection. Tested with WB, IHC-P in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 2735
Other Names Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI, GLI1, GLI
Calculated MW 117904 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, By Heat

Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
Tissue Specificity Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. .
Protein Name Zinc finger protein GLI1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E.coli-derived human GLi1 recombinant protein (Position: Q768-A1106). Human GLi1 shares 79% amino acid (aa) sequence identity with mouse GLi1.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the GLI C2H2-type zinc-finger protein family.
Protein Information
Name GLI1
Synonyms GLI
Function Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24076122, PubMed:24311597, PubMed:24217340). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340). Regulates the transcription of specific genes during normal development (PubMed:19706761). Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761, PubMed:28973407). Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed:11238441, PubMed:28973407).
Cellular Location Cytoplasm. Nucleus. Note=Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441)
Tissue Location Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761)
Research Areas
Citations (0)
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Background

GLI1, also known as GLI, is a protein originally isolated in human glioblastoma. The protein is encoded by GLI1 gene. It is mapped to 12q13.3. This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Beside, various upstream patterning signals may be integrated by the Gli proteins to direct a coherent programme of neurogenesis.

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$ 280.00
Cat# ABO11814
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