Anti-GATA5 Picoband Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q9BWX5 |
Host | Rabbit |
Reactivity | Human, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Transcription factor GATA-5(GATA5) detection. Tested with WB in Human;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 140628 |
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Other Names | Transcription factor GATA-5, GATA-binding factor 5, GATA5 (HGNC:15802) |
Calculated MW | 41299 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Rat, Human |
Subcellular Localization | Nucleus . |
Protein Name | Transcription factor GATA-5 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen | E.coli-derived human GATA5 recombinant protein (Position: M1-A397). Human GATA5 shares 75% amino acid (aa) sequence identity with mouse GATA5. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Sequence Similarities | Contains 2 GATA-type zinc fingers. |
Name | GATA5 (HGNC:15802) |
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Function | Transcription factor required during cardiovascular development (PubMed:23289003). Plays an important role in the transcriptional program(s) that underlies smooth muscle cell diversity (By similarity). Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer (PubMed:25543888). |
Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Transcription factor GATA-5, also named GATAS or GATA5, is a protein that in humans is encoded by the GATA5 gene. It is mapped to 20q13.33. The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. In addition, the expression of GATA5 increased in a human colon cancer cell line within 2 hours of exposure to sodium butyrate, which induces differentiation.
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