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Anti-Vimentin Picoband Antibody

     
  • WB - Anti-Vimentin Picoband Antibody ABO12050
    Anti- Vimentin Picoband antibody, ABO12050, Western blottingAll lanes: Anti Vimentin (ABO12050) at 0.5ug/mlLane 1: HT1080 Whole Cell Lysate at 40ugLane 2: NIH Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: HUT Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: Human Placenta Tissue Lysate at 50ugLane 8: Rat Testis Tissue Lysate at 50ugLane 9: Mouse Testis Tissue Lysate at 50ugPredicted bind size: 54KDObserved bind size: 54KD
  • IHC - Anti-Vimentin Picoband Antibody ABO12050
    Anti- Vimentin Picoband antibody, ABO12050, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue
  • IHC - Anti-Vimentin Picoband Antibody ABO12050
    Anti- Vimentin Picoband antibody, ABO12050, IHC(P)IHC(P): Rat Cardiac Muscle Tissue
  • IHC - Anti-Vimentin Picoband Antibody ABO12050
    Anti- Vimentin Picoband antibody, ABO12050, IHC(P)IHC(P): Human Mammary Cancer Tissue
  • WB - Anti-Vimentin Picoband Antibody ABO12050
    Anti- Vimentin Picoband antibody, ABO12050, Western blottingAll lanes: Anti Vimentin (ABO12050) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 54KDObserved bind size: 54KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession P08670
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Vimentin(VIM) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Calculated MW 53652 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human, Mouse, Rat
Subcellular Localization Cytoplasm .
Tissue Specificity Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. .
Protein Name Vimentin
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin (435-466aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the intermediate filament family.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
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Background

VIM(vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

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$ 280.00
Cat# ABO12050
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